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In 1971 Brown and Dryburgh unravelled the complete sequence of porcine GIP

Methods Materials Tetrahydrofuran (THF), toluene and ethanol were distilled from sodium benzophenone ketyl, calcium hydride and magnesium, respectively, under nitrogen immediately prior to use

High pH fractionation- TMT labeled, combined, and dried samples were resuspended in 300 L of Buffer A (5% v/v MeCN, 95% v/v H 2 O, 0.1% v/v formic acid) and fractionated by centrifugation using a peptide desalting column (Pierce, #89852)

KPV, Anti-Inflammatory Tripeptide KPV (Lys-Pro-Val) is the C-terminal tripeptide of alpha-MSH

Eligibility varies based on personal health factors and clinical judgment